| Bristol Rovers2-0Cambridge United | 90 minutes | Bristol Rovers: R. Russell-Denny 57', F. Cavegn 72' | Cambridge's Sullay Kaikai blasted an 82nd-minute penalty against the Bristol Rovers crossbar, and with it went the League One side's best route back from two-nil down. |
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| Oxford City2-4Leicester City | 90 minutes | Oxford City: S. Pollock 31', J. Ashby 62'Leicester City: L. Page 16', E. Riis 53', K. Doyle 59', T. Watson 82' | Leicester's half-time substitute Harry Howell crossed for team-mate Emil Riis and started the move for team-mate Kamari Doyle's goal, turning a tie Oxford City had level at the break. |
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| Accrington Stanley0-2Wycombe Wanderers | 90 minutes | Wycombe Wanderers: C. Taylor 5', D. Harvie 69' | Wycombe's Connor Taylor followed up his own blocked shot from a fifth-minute corner, and team-mate Daniel Harvie headed in another corner to finish off League Two Accrington. |
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| Barnet3-0Tranmere Rovers | 90 minutes | Barnet: K. Smith 80', N. Ofoborh 90', D. Ajayi 90+6' | Barnet's three goals all came from substitutes from the 80th minute on, after their goalkeeper Matthew Cox had twice denied Tranmere's Charlie Whitaker. |
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| Barnsley7-2Aldershot Town | 90 minutes | Barnsley: C. McGeehan 20', R. Cleary 26', V. Yoganathan 33', V. Yoganathan 45+2', C. McGeehan 62', C. McGeehan 67', R. Brownlie 87'Aldershot Town: Annisuis Lewis 75', Annisuis Lewis 86' | Barnsley led four-nil at the break and finished with a Cameron McGeehan hat-trick, while Aldershot substitute Annisuis Lewis chipped in both goals for the visitors. |
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| Boreham Wood0-3Wigan Athletic | 90 minutes | Wigan Athletic: J. Mellish 11', M. Fox 36', M. Fox 74' | Wigan's Morgan Fox scored from a 25-yard free-kick and headed in team-mate Max Power's corner, inflicting the campaign's first home defeat on Boreham Wood. |
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| Bradford City7-0Marine | 90 minutes | Bradford City: N. Powell 10', N. Powell 16', A. Phillips 21', M. Pennington 33', W. Swan 58', G. Lapslie 73', A. Phillips 87'Sent off: I. Abankwah 54' (Marine) | Marine lost Isaac Abankwah to a straight red for conceding a 54th-minute penalty, and though team-mate Jack McIntyre saved it with his legs, Bradford already led four-nil. |
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| Bromley0-2Rotherham United | 90 minutes | Rotherham United: S. Aljofree 45+1', T. Bradshaw 56' | Bromley's Jacob Mendy erred for Rotherham's second goal, and a side winning for the first time in 10 matches spoiled Karl Robinson's start as Bromley manager. |
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| Burton Albion4-0AFC Rushden & Diamonds | 90 minutes | Burton Albion: W. Collar 23', R. Borges Rodrigues 35', C. Webster 75', Z. Scutt 90+5' | Burton kept AFC Rushden & Diamonds striker Billy Sharp from a 300th career goal, and the tier-eight visitors managed two shots on target all afternoon. |
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| Cheltenham Town2-0Halifax Town | 90 minutes | Cheltenham Town: J. Evans 16', G. Miller 90+3' | Halifax's Conor Carty had a 63rd-minute goal ruled out for offside, and Cheltenham substitute George Miller headed in team-mate Charlie McCann's corner in added time to end it. |
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| Chesterfield4-3Yeovil Town | 90 minutes | Chesterfield: J. Simpson 43', J. Koroma 45', J. Simpson 55', J. Randall 89'Yeovil Town: S. Spasov 38' (pen), J. Daly 78', H. Muller 82' | Yeovil came back from three-one down to level at three-all, only for Chesterfield substitute Joel Randall to win it a minute from time. |
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| Colchester United1-0Worcester City | 90 minutes | Colchester United: J. Williams 32' | Colchester's Jaden Williams scored in the 32nd minute, and Worcester City goalkeeper Cristiano Dzialuk's six saves, one a point-blank stop from Colchester's Oscar Thorn, kept it there. |
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| Crewe Alexandra2-1AFC Wimbledon | 90 minutes | Crewe Alexandra: C. McNeill 12', C. Thomas 62'AFC Wimbledon: A. Yiadom 82' | Crewe's Charlie McNeill beat AFC Wimbledon goalkeeper Josef Bursik, who had strayed from his line, from halfway, then saw his free-kick parried for team-mate Conor Thomas to head in. |
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| Dover Athletic2-0Wealdstone | 90 minutes | Dover Athletic: J. Bates 14', J. Bates 42' | Dover's Jack Bates struck twice from outside the box before half-time, enough for the tier-six hosts against tier-five Wealdstone despite only 33% of the ball. |
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| Eastleigh1-2Shrewsbury Town | 90 minutes | Eastleigh: W. Randall-Hurren 79'Shrewsbury Town: G. Lloyd 38' (pen), I. Fletcher 87' | Shrewsbury substitute Isaac Fletcher tapped in team-mate Tom Sang's cross with three minutes left, after Eastleigh had claimed the foul for Shrewsbury's penalty was outside the area. |
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| Guiseley1-0Sutton United | 90 minutes | Guiseley: J. Thewlis 90+5' (pen) | Guiseley substitute Jordan Thewlis converted a stoppage-time penalty to win a tie whose second half started around 15 minutes late after a medical incident among the spectators. |
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| Harrogate Town1-4Fylde | 90 minutes | Harrogate Town: W. McGowan 9'Fylde: L. Thomas 16', A. Drewe 60', T. Whelan 78' (pen), M. Matthews-Lewis 82' | AFC Fylde, level with their fellow National League hosts until the hour, went ahead then and scored twice more in the last 12 minutes. |
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| Huddersfield Town1-2Exeter City | 90 minutes | Huddersfield Town: I. Bowat 54'Exeter City: J. Taylor 12', C. Cummins 81' | Exeter's Charlie Cummins finished team-mate Reece Cole's cutback nine minutes from time as the tier-four visitors won at a tier-three Huddersfield side showing six changes. |
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| Leyton Orient5-1Harborough Town | 90 minutes | Leyton Orient: A. Dobra 6', A. Connolly 25', I. El Mizouni 50', T. Springett 81', I. El Mizouni 90+6'Harborough Town: P. Malone 69' | Leyton Orient had 20 shots to seven and led two-nil at the break, with Harborough substitute Paul Malone's turned-in corner the tier-six visitors' only reply. |
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| Luton Town2-0Forest Green Rovers | 90 minutes | Luton Town: E. Lawrence 4', J. Clark 41' | Forest Green Rovers had 17 shots to Luton's eight but only two on target, and the tier-three hosts were two goals up by the 41st minute. |
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| MK Dons2-1Swindon Town | 90 minutes | MK Dons: M. Ekpiteta 6', A. Collins 13'Swindon Town: A. Drinan 45+1' | MK Dons were two up by the 13th minute, Aaron Collins scoring his 17th of the season, and home goalkeeper Craig MacGillivray later denied Swindon's Aaron Drinan an equaliser. |
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| Mansfield Town3-0Macclesfield | 90 minutes | Mansfield Town: N. Moriah-Welsh 21', R. Oates 73', J. Bowery 90+3' | Mansfield's Rhys Oates bundled in team-mate Michael Smith's far-post cross in the 73rd minute, and only then was tier-six Macclesfield's resistance over. |
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| Newport County3-1Walsall | 90 minutes | Newport County: K. Etete 19', M. Adu-Poku 23', K. Etete 60'Walsall: A. Dallas 7' | Behind to Walsall after seven minutes, Newport County scored twice in four minutes, and Kion Etete's second meant Walsall lost away for the first time this season. |
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| Oxford United0-1Stevenage | 90 minutes | Stevenage: C. Piergianni 4' | Stevenage's Carl Piergianni headed in team-mate Ben Broggio's cross against an Oxford United side showing nine changes, and Stevenage goalkeeper Tommy Setford tipped over a header from Oxford's Gatlin O'Donkor. |
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| Peterborough United0-5Reading | 90 minutes | Reading: D. Kyerewaa 26', J. Marriott 34', C. Savage 45+4', J. Reid 72', J. Reid 76' | Reading's Charlie Savage opened his account for the season against a Peterborough side managed by his dad Robbie, and Reading led three-nil at the break. |
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| Plymouth Argyle4-0Alvechurch | 90 minutes | Plymouth Argyle: W. Evans 50', W. Evans 69', M. Baidoo 78', R. Curtis 80' | Goalless at the break against tier-seven Alvechurch, Plymouth Argyle led through Will Evans on 50 minutes, then added three goals inside 11 minutes late on. |
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| Port Vale4-2Scunthorpe United | 90 minutes | Port Vale: K. Figueroa 8', O. Hernández 58', M. Watters 61', O. Lynch 90+5'Scunthorpe United: J. Norwood 86', D. Howe 90' | Three goals up after 61 minutes, Port Vale let Scunthorpe back to three-two through James Norwood and Declan Howe before Port Vale substitute Oli Lynch's stoppage-time goal settled it. |
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| Salisbury6-0Sholing | 90 minutes | Salisbury: N. Odokonyero 31', J. Hedges 33' (pen), J. Sivi 39', N. Odokonyero 53', L. Russe 89', J. Bartolo 90+3' | Salisbury, never in the second round before, got there as Josh Hedges won and converted the 33rd-minute penalty against tier-seven Sholing. |
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| York City4-0Northampton Town | 90 minutes | York City: O. Pearce 1', O. Pearce 11', A. Hunt 29', J. Stones 81' | York City captain Ollie Pearce scored after 10 seconds, and Northampton goalkeeper Zach Jeacock, hurt colliding with a post for that goal, was swiftly substituted. |
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